Question

You have a piece of DNA containing the gene for green fluorescent protein (gfp) and the pBAD promoter. This DNA fragment has been cleaved with BamHI and HindIII and can be inserted into either the pKAN-R plasmid or the pARA plasmid. Which plasmid would you choose to use as the plasmid vector to express gfp protein in bacterial cells

Answer 1

pARA plasmid

Step-by-step explanation:

Plasmids are natural circular DNA samples found in bacteria cells. There are two types of plasmids founds in bacteria cells namely; pARA and pKAN-R.

The pKAN would not be maintained in the cell during replication but the pARA plasmid would and when the DNA fragment is inserted, the pARA would serve as a catalyst for the gfp protein.