Question

The reasons for using transposon-based mutagenesis include all of the following EXCEPT ________. A) likely to cause only minimal effects on gene function B) antibiotic selection can be employed during mutagenesis. C) mutations are often 'tagged' by the nearby transposon D) dramatic mutations which often cause null alleles E) transposons are found in nearly all organisms

Answer 1

A) likely to cause only minimal effects on gene function

Explanation:

Transposon based mutagenesis is a versatile technique used in molecular biology laboratories in order to make gene functional screening on a genome-wide scale. This technique is particularly useful in species that are difficult to transform because transposon-based mutagenesis systems do not require transformation. The transposon-based mutagenesis systems produce mutations capable of disrupting gene function via insertions in or near genes. Moreover, it is important to note that transposon-based mutagenesis triggers multiple insertions in the genome, thereby may be difficult to identify the mutation that is associated with a particular phenotype.