Answer:
D. E. Coli
Step-by-step explanation:
Prokaryotes are single-celled organisms that lack nuclei and membrane-bound cell structures. The two domains that are classified as prokaryotic are Eubacteria and Archaea.
E. Coli is a bacteria, belonging to the domain Eubacteria, therefore it is a prokaryote.
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To eliminate the other answer choices, portabella mushrooms, roses, and butterflies are all eukaryotic organisms. (Eukaryotes are all living things and fungi, besides eubacteria and archaebacteria.)