Answer:
Volume of stock 50X TAE buffer required is 4 mL;
Volume of distilled water needed = 196 mL
The researcher will measure 4 mL of the stock 50X TAE solution using a pipette into a 250 mL volumetric flask. The researcher will then add 196 ml of distilled water to make it up to the 200 ml mark.
Step-by-step explanation:
The following values are given: initial concentration, final concentration and final volume
Initial concentration,C₁ = 50X
Final concentration, C₂ = 1X
Final volume, V₂ = 200 mL
Initial volume, V₁ = ?
Using C₁V₁ = C₂V₂
V₁ = C₂V₂/C₁
V₁ = 1X * 200 mL/50X
V₁ = 4 mL
Therefore, volume of stock 50X TAE buffer required is 4 mL
Volume of distilled water needed = 200 mL - 4 mL
Volume of distilled water needed = 196 mL
The researcher will measure 4 mL of the stock 50X TAE solution using a pipette into a 250 mL volumetric flask. The researcher will then add 196 ml of distilled water to make it up to the 200 ml mark.