Question

What common features do eukaryotic mRNA share? Please use one of these features to describe how you can isolate eukaryotic mRNA from the cell extract. (limit your answers in 4 lines)

Answer 1

A common feature in mRNAs that allow extraction from the cell is the 5' cap region which has a Guanine nucleotide attached to the mRNA in an atypical five prime to five prime triphosphate linkage.

Step-by-step explanation:

The 5' cap structure serves to protect the mRNA against damage from RNA phages and an extraction protocol an be used to isolate the mRNAs from the cells. Reagents such as Dichloromethane, sodium hydroxide and diethyl ether in aqueous solution helps separate the organic layer, a silica-gel column can be used to further isolate the mRNAs