Answer:
Obligate aerobic microbes requires oxygen for its survival in the thioglycolate tube while Obligate anaerobic microbes does not requires oxygen.
Step-by-step explanation:
Thioglycolate tube is a tube which is used to determine the oxygen requirement of different types of microbes. The concentration of oxygen is higher in the upper portion of the tube while in the lower portion, the concentration of oxygen is the lowest. So those microbes who needs oxygen for their survival will be present in the upper portion of the tube while those microbes who does not need oxygen, will be present at the bottom of the tube.