Question

Students have expressed a protein according to the BASIL protocols. They collected a pre-induction sample, a post-induction sample, and a sample following an affinity column purification. The samples were analyzed by SDS-PAGE followed by Coommassie Blue staining. Which lane corresponds to which sample?

Answer 1

The first lane with the protein band is the post-induction sample, i.e., the lane with the sample of interest.

Step-by-step explanation:

Antibody binding is specific and will only bind to whichever protein it has been produced for particularly, which in this case would be the post induction sample.