Final answer:
Unicellular organisms perform all life functions within a single cell, lacking differentiated tissues and organs found in multicellular organisms.
Step-by-step explanation:
The characteristic of unicellular organisms that distinguishes them from multicellular organisms is that all of their functions are performed by a single cell. Unlike multicellular organisms, which are organized into tissues, organs, and organ systems with specialized cells that perform specific functions, unicellular organisms must carry out every process necessary for life within that single cell. Although unicellular organisms can be complex and have highly organized internal structures, they do not have true differentiated tissues and organs like multicellular organisms do.