Question

Your research group is investigating the possible use of genetically - engineered cells to produce a vaccine for malaria. In paragraph form, explain the pros AND cons of using E. coli, Saccharomyces, AND animal cells to produce the vaccine.

Answer 1

Attributes of E. coli articulation frameworks

Advantages:

• Quick articulation
• Simplicity of culture
• Significant returns
• Cheap
• Genome alterations conceivable

• Large scale manufacturing quick and practical

Disadvantages:

• Proteins with disulfide bonds hard to communicate
• Produce unglycosylated proteins
• Proteins created with endotoxins
• Acetic acid derivation development bringing about cell lethality
• Proteins created as consideration bodies
• produce dormant proteins
• needs collapsing

YEAST SYSTEM

Advantages:

• Nearness of post translational change
• discharge can be recognized by emission signal
• develop in minimal effort media
• straightforward hereditary control

Disadvantages:

• Low yeild of item

Bacillus articulation frameworks

Advantages:

• Solid discharge
• no association of intracellular consideration bodies
• Simplicity of control
• Hereditarily all around portrayed frameworks
• Exceptionally created change and quality substitution advancements.
• Unrivaled development qualities
• financially savvy recuperation

Animal Cells:

Advantage:

• nearness of post interpretation adjustment

Disadvatages

Issues with creature utilization

Can get sullied with creature diseases

Exorbitant downstream preparing