Question

If one were to cut the plasmid DNA with Hindill, Apal, and Smal, how large is each

segment produced?

Answer 1

A linear shaped piece of DNA strand is obtained when a plasmid is cut HindIII, Apa1, and Sma1.

Step-by-step explanation:

Restriction enzymes are endonucleases which enzymatic proteins which can cut a DNA at specific sequences at particular recognition sites. The sequences recognized by these enzymes are usually palindromic that occurs for a definite length (6 bp, 4 bp, or 8 bp).

They can either be blunt cutters or sticky end cutters. APA1, SMA1, and HindIII are all restriction enzymes which can cut a circular plasmid at specific locations.

These restriction enzymes bind with the DNA at specific locations and cuts them to give linear strands of DNA either with a blunt or a sticky end.