Answer: Peptidoglycan layer
Step-by-step explanation:
Based on gram stain, bacteria are classified as:
- Gram positive, having thick Peptidoglycan layer that retains more dye/stain after being rinsed by alcohol. Examples are staphylococcus Aureus, Clostridium etc
- Gram negative, having thin Peptidoglycan layer that retains less dye/stain after being rinsed by alcohol. An example is Escherichia coli
Thus, the difference in Peptidoglycan layer helped group bacteria into two