Question

Below is a fragment of a DNA strand. A single primer is pictured below the strand in the gray box. 3'-ATACGACTAGTCGGGACCATATC-5'… 5’-TATGCTG Draw the resulting sequencing gel from a dideoxy sequencing reaction, using a single column for each of the four bases.

Answer 1

Answer: 5'-TATGCTGATCAGCCCTGGTATAG-3'

Step-by-step explanation:

DNA consists of 4 nucleotides; Adenine (A), Thymine (T), Cytosine (C) and Guanine (G), which serve as it's building block. The nucleotides are linked by 3', 5' phosphodiester bonds.

Phosphodiester bonds are bonds that join two nucleotides together.

Guanine (G) which always pairs with Cytosine (C) and Adenine (A) always pairs with Thymine (T). Percentage of A usually equals that of T while that of C usually equals G. This is known as Chargaff's rule.

So, to write the sequence of the complementary strand, you interchange the nucleotides with their corresponding partner.