Question

When ejecting a protein sample into a well of the SDS-PAGE gel with a pipetter, why should you only push down on the pushbutton to the first stop and not the second stop?Select one:

a. Pushing down to the second stop will eject more sample than required.
b. The pushbutton should only be pushed down to the second stop when loading the sample into the pipetter.c. Pushing down to the second stop when loading the samples will not affectthe SDS-PAGE results.d. Pushing down to the second stop could denature the protein sample.e. Pushing down to the second stop will result in ejection of air from the pipet tip, which could blow the content intended for that particular well to the surrounding wells

Answer 1

.e. Pushing down to the second stop will result in ejection of air from the pipette tip, which could blow the content intended for that particular well to the surrounding wells

Step-by-step explanation:

The pipette has two stops the first and the second stop. The first stop is the one that changes in other words the amount that is set. The second stop is for extra push to remove all the liquid from the tip. When the pipette is pushed to the second stop some air is ejected.

This is the main reason when loading samples on wells during SDS-PAGE ,we stop at the first stop or else the contents will go to the neighboring wells.