Question: Did deletion of any of the possible control elements cause a reduction in reporter gene expression? if so, which one(s), and how can you tell?
None of the deletions caused a reduction in reporter gene expression; all of them still resulted in reporter mRNA being made.
Deletion of elements #2 and #3 caused a reduction in reporter gene expression; those constructs resulted in less than the highest level of mRNA.
Deletion of element #3 caused a reduction in reporter gene expression; that construct resulted in less than 50% of the control level of mRNA
Answer:
Deletion of any of the possible control elements cause a reduction in reporter gene expression because "deletion of element #3 caused a reduction in reporter gene expression; that construct resulted in less than 50% of the control level of mRNA."
Step-by-step explanation:
Yes, the removal of the possible control elements which will further cause in the reduction of reporter gene expression. As a result of which the deletion of the control element, or we may say the activator will not be able to bind to the enhancer. Which on subsequent manner will lead to the reduction of gene expression. Various factors affects the gene expressions like food, certain drugs, coming in contact to various toxins, which leads to the epigenetic changes as due to changing the way by which the molecule gets attached.