Question

15. Assuming the fluorescence threshold is set to be 1. Which gene has the highest gene expression rate? What is the Ct value? Gene with highest expression = ______________ Ct value = _______________

Answer 1

The correct answer is - Gene 1 and 14.5 (or 14).

Step-by-step explanation:

The cycle threshold is denoted as Ct value. It is the cross point at the threshold line meets with fluorescence signal reaction curve. In the given RTpcr experiment, the threshold line is fluorescence intensity 1 thus, Ct value for each gene will be the point at which each curve meets the fluorescence intensity line at 1. Ct value of is inversely proportional to amount of nucleic acid in the given sample.

Here, the Ct for gene 1 is the lowest, so the amount of DNA would be highest.

Gene with highest expression= Gene 1

Ct value= 14.5