Question

An ampicillin-resistant, tetracycline-resistant plasmid, pBR322, is cleaved with PstI, which cleaves within the ampicillin resistance gene. The cut plasmid is ligated with PstI digested Drosophila DNA to prepare a genomic library, and the mixture is used to transform E. coli K12. Which Antibiotic should be added to the medium to select cells that have incorporated a plasmid

Answer 1

Ampicillin should be added to the medium to select cells that have incorporated a plasmid.

Step-by-step explanation:

The medium that should be added to select cells that have incorporated a plasmid is ampicillin. The plasmid pBR322 used in this experiment contains an ampicillin resistance gene, which allows the cells harboring the plasmid to survive in the presence of ampicillin. By adding ampicillin to the growth medium, only the transformed cells containing the plasmid pBR322 will be able to grow, while the non-transformed cells will not.