Question

what absorbance did you record for spinach at 400 nm?6.25 points question 2what absorbance did you record for spinach at 420 nm?6.25 points question 3what absorbance did you record for spinach at 440 nm?

Answer 1

The exact absorbance values for spinach at 400 nm, 420 nm, and 440 nm are not provided. Therefore, it is not possible to determine these values without additional information.

Step-by-step explanation:

Based on the information provided, the absorbance recorded for spinach at different wavelengths is not given. However, it is mentioned that the absorbance value at 260 nm (A260) is about 1.0 in the given spectrum for NAD*.

Since the absorbance values for spinach at 400 nm, 420 nm, and 440 nm are not provided, it is not possible to determine the exact absorbance values at these specific wavelengths for spinach.

To calculate the concentration of a sample, the Beer-Lambert Law and the molar extinction coefficient are used. However, the necessary information is not given to perform this calculation for spinach.