Question

Describe the process of cloning from start to finish.

Answer 1

Step-by-step explanation:

• In standard molecular cloning experiments, the cloning of any DNA fragment essentially involves seven steps: (1) Choice of host organism and cloning vector, (2) Preparation of vector DNA, (3) Preparation of DNA to be cloned, (4) Creation of recombinant DNA, (5) Introduction of recombinant DNA into host organism, (6) ...

Answer 2

1. Isolation of donor DNA fragment or gene.

2. Selection of suitable vector.

3. Incorporation of donor DNA fragment into the vector.

4. Transformation of recombinant vector into a suitable host cell.

5. Isolation of recombinant host cell.