Complete question:
(C) Fill in the boxes in the template to identify the nucleotide sequence, from 3' to 5´ of the tRNA anticodon that will recognize codon 5 and the tRNA anticodon that will recognize codon 510 of the mRNA template and to identify the amino acids that will be added at these two positions.
In the attached files you will find the image
Answer:
Codon Anticodon Amino Acid
5 CGG → GCC → Arginine, Arg
510 UAG → AUC → Stop
Step-by-step explanation:
Some theory that you need to know to answer the question:
Each tRNA has two important sites. One of them that couples with the codon of the mRNA molecule, named anticodon. The other site couples with an amino acid through the action of the aminoacyl-tRNA synthase enzyme. The whole complex, amino acid + enzyme + tRNA is named aminoacyl-tRNA. Considering that there are twenty amino acids available, there are also twenty complexes of aminoacyl-tRNA, one for each amino acid.
tRNA allows amino acids to align according to the nucleotidic sequence in the mRNA molecule.
Once the new peptidic link joins, placing together the new amino acid to the growing peptidic chain, the binding between the amino acid and the tRNA molecule breaks. The tRNA is now free to join another amino acid and repeat the cycle.
Now, to fill in the boxes, you need to pay attention to two things:
- Anticodon: In this box, you need to place the sequence of bases that pairs with the mRNA codon.
- Amino Acid: In this box, you need to place the amino acid that is codified by the mRNA codon.
For instance, let us say that the codon of mRNA is CUC.
The anticodon for this sequence is GAG (because guanine joins cytosine and adenine joins uracyl).
The amino acid placed on the 3´extreme of the tRNA molecule will be the one that is codified by the codon, NOT the anticodon. So, as the codon is CUC, the amino acid will be Leucine, Leu. Do not use the anticodon GAG to fill these boxes. Remember that tRNA is translating information carried by the mRNA, so we need to use this information to grow the protein.
So, in the problem, there are two codons: 5 and 510
Codon Anticodon Amino Acid
5 CGG → GCC → Arginine, Arg
510 UAG → AUC → Stop