Question

For the following template

DNA sequence:
3 TÁC AGA - GGT -
TCG - ATC 5'
a) provide the amino acid
sequence
b) provide the anticodons in
order
c) Mutate the above DNA
sequence to introduce a
silent substitution. Write the
mutated DNA sequence
down.
Make sure to clearly
indicate if your answer for
each part by labeling it a) or
b) or c)

Answer 1

a) Methionine- Serine- Proline- Serine- STOP.

b) UAC- AGA - GGU -

UCG - AUC

c) AUG- UCG- CCA- AGC- UAG

Step-by-step explanation:

A) The DNA sequence in this question is given as follows: 3 TÁC -AGA - GGT -

TCG - ATC 5'. This sequence undergoes transcription and translation (gene expression) in order to give rise to an amino acid sequence.

The mRNA sequence that will result following transcription is: AUG- UCU- CCA- AGC- UAG

Following translation, each codon in the above mRNA sequence will result in the following amino acid sequence: Methionine- Serine- Proline- Serine- STOP.

B) Anticodons are a group of three nucleotides that are complementary to a codon found on the tRNA molecule. The anticodons for each codon on the mRNA sequence is: UAC- AGA - GGU -

UCG - AUC

C) Silent mutation is a type of substitution mutation in which one or more nucleotide base is replaced by another in such a way that it does not change or alter the amino acid sequence. Using the mRNA sequence: AUG- UCU- CCA- AGC- UAG

A mutated sequence is as follows: AUG- UCG- CCA- AGC- UAG. Note that, G has replaced U in the second nucleotide. However, there is no change in amino acid that results because the same UCU that codes for SERINE is also encoded by UCG.