Question

The composition of PBS is 0.137M NaCl, 0.012M Phosphate, 0.0027M KCl, pH 7.4. Below is the protocol to make 1 litre of 10x concentrate PBS.Combine the following:•80g NaCl•2g KCl•14.4g Na2HPO4 (dibasic anhydrous)•2.4g KH2PO4 (monobasic anhydrous)•800mL distilled H2O1.Adjust pH to 7.4 with HCl2.Add H2O to 1L3.Autoclave for 20 minutes on liquid cycle. Store at room temperature.Which ions are being produced by this process, assuming that each of the chemical compounds dissociate into their constituent parts once they are dissolved in water?

Answer 1

The ions produced by the process of preparing the solutions are:

• Na+

• Cl-

• K+

• H+

• PO4 3-

,

• OH-