Question

Given 200ul of a 0.5mg/ml stock solution of BSA, how much do you pipet into a test tube so that you are adding 5ug of BSA to the test tube

Answer 1

Answer:
`10\mu L` of volume needs to be pipetted out in the test tube.

Step-by-step explanation:

We are given:

Mass of BSA to be formed =
`5\mu g=0.005mg` (Conversion factor:
`1mg=1000\mu g`

Volume of stock solution =
`200\mu L=0.2mL` (Conversion factor:
`1mL=1000\mu L`

It is also given that for the mass of BSA is 0.5 g, the volume used up is 1 mL

In order to have, 0.005 g, the volume of stock solution needed will be =
`(1mL)/(0.5g)* 0.005g=0.01mL=10\mu L`

Hence,
`10\mu L` of volume needs to be pipetted out in the test tube.