Question

Rosalyn Yalow received the Nobel prize for the development of a highly sensitive analytical method that has tremendous application in hormone assays as well as assays of many other substances found at low levels in biological fluids. The assay employs isotypically labeled molecules and permits measurements of extremely small amounts of antigen, antibody, or antigen-antibody complexes. What is this assay called?

a) PCR
b) RIA
c) IEF
d) SDS-PAGE
e) FACS

Answer 1

Option (b), The assay developed by Rosalyn Yalow that received the Nobel Prize is called radioimmunoassay (RIA), which uses radioisotopes to detect small quantities of antigens in biological fluids.

Step-by-step explanation:

Rosalyn Yalow received the Nobel Prize for developing the radioimmunoassay (RIA), a highly sensitive analytical technique. The technique relies on radioisotopes to detect and measure small quantities of antigens in biological fluids.

It operates on the principle of competitive binding, where radioactively labeled antibodies are used to quantify the amount of antigen present. Thanks to its high sensitivity, RIA is utilized in various fields including endocrinology, narcotics detection, blood bank screening, early cancer diagnosis, hormone measurement, and allergy diagnosis.