Question

What must you be sure to add to a transgene before inserting into a mouse line (knock-in)?

1) Promoter sequence
2) Enhancer sequence
3) Reporter gene
4) All of the above

Answer 1

To create a knock-in mouse line, you must add a promoter sequence, an enhancer sequence, and a reporter gene to the transgene, meaning 'All of the above' is the correct answer.

Step-by-step explanation:

When creating a knock-in mouse line with a transgene, it is essential to add several elements to ensure proper gene expression. These include a promoter sequence, which initiates transcription; possibly an enhancer sequence to increase the gene's expression level; and a reporter gene to confirm the insertion of the transgene. Therefore, the correct answer to what you must add to a transgene before inserting it into a mouse line is 4) All of the above.