Question

In order to ________, macromolecules and RNA must be destroyed.

Answer 1

To extract nucleic acids and RNA, cells are broken open using a lysis buffer and macromolecules are inactivated using enzymes such as proteases and RNAses. DNA can be precipitated using alcohol.

Step-by-step explanation:

Most nucleic acid extraction techniques involve steps to break open the cell and use enzymatic reactions to destroy all macromolecules that are not desired (such as degradation of unwanted molecules and separation from the DNA sample). Cells are broken using a lysis buffer, which is mostly a detergent. These enzymes break apart lipid molecules in the cell membranes and nuclear membranes. Macromolecules are inactivated using enzymes such as proteases that break down proteins, and ribonucleases (RNAses) that break down RNA. The DNA is then precipitated using alcohol.

Answer 2

In order to synthesize (build or create), macromolecules and RNA must be destroyed (broken down).