Final answer:
MS tissue must be retrieved aseptically and kept at a temperature that prevents microbial growth without freezing the tissue, typically at refrigeration temperatures (0 °C to 7 °C), so none of the provided answers is ideal. However, 10 °C is the closest to the correct temperature range, which suggests a typo in provided options. Option D is correct.
Step-by-step explanation:
The question asks about the maximum temperature at which refrigerated MS tissue should be retrieved aseptically and placed in an isotonic or nutrient medium with suitable antibiotics.
While the question does not explicitly state the correct answer, we can infer from standard laboratory protocols and industry knowledge that refrigerated samples are typically kept at temperatures that inhibit microbial growth but do not freeze the tissue.
Refrigerators used in a laboratory setting maintain temperatures between 0 °C and 7 °C. For immediate processing, tissues are often brought to a temperature that is still cold enough to prevent microbial growth but not so cold as to damage the tissue or impede certain laboratory processes, which typically would be above the standard refrigeration range but still well below room temperature.
Considering the options provided and common laboratory practices, the MS tissue should be placed in a medium with suitable antibiotics at no more than 4 °C to ensure both the preservation of the tissue and the inhibitory effect on potential microbial contamination.
Since this temperature is not listed as an option, it could be a typographical error in the question, as long-term storage temperatures are not suitable for immediate processing, which typically happens at refrigeration temperatures. Thus, none of the options given (15 °C, 20 °C, 25 °C, 10 °C) are ideal, but 10 °C might be chosen as an answer closest to the standard preservation range in absence of the correct option.