Question

Which direction does DNA pol III build?

A) 5' to 3'
B) 3' to 5'
C) Bidirectional
D) Random

Answer 1

DNA polymerase III builds DNA in the 5' to 3' direction. Phosphodiester linkages are formed when nucleotides are added to the new strand, and Okazaki fragments are synthesized on the lagging strand in a discontinuous manner due to this directionality. option A is answer

Step-by-step explanation:

DNA polymerase III synthesizes DNA by adding nucleotides to the growing DNA strand. The direction in which DNA polymerase III can build is strictly from the 5' to 3' end. This represents the addition of nucleotides to the 3' end of the new strand, which grows in the 5' to 3' direction. The template strand, therefore, is read in the opposite 3' to 5' direction to enable the correct alignment of nucleotides.

An important aspect of DNA replication is the synthesis of what are called Okazaki fragments on the lagging strand. These are short, newly synthesized DNA segments that are formed because the DNA polymerase can only add nucleotides in the 5' to 3' direction, which leads to a need for discontinuous replication on one of the two strands being synthesized. option A is answer