Step-by-step explanation:
Restriction Enzyme Digestion: DNA can be cut into fragments using restriction enzymes, which recognize specific sequences of nucleotides and cut the DNA at those sites.
2. PCR Amplification: DNA fragments can be amplified using the polymerase chain reaction (PCR). This technique uses a DNA polymerase enzyme to make multiple copies of a specific DNA sequence.
3. Chromosome Walking: This technique involves using a primer to amplify a specific DNA sequence and then using the amplified product as a template for the next round of amplification. This process continues until the entire region of interest is amplified.