Question

All of the DNA fragments will be______ in charge, migrate toward the_______ differences in and separate from one another due to differences in_______

Choose one answer from below.
a. negative; cathode; size
b. negative; anode; charge
c. negative; anode; size
d. positive; cathode; size
e. positive; cathode; charge

Answer 1

The correct answer is c. negative; anode; size. DNA fragments are negatively charged and separate in a gel when an electric field is applied, migrating towards the positive electrode (anode) and are separated by size.

Step-by-step explanation:

All of the DNA fragments will be negative in charge, migrate toward the anode, and separate from one another due to differences in size. Therefore, the correct answer is c. negative; anode; size. DNA molecules are negatively charged due to the phosphate groups in the DNA backbone, and in gel electrophoresis, they are placed in a gel with an electric field applied.

The DNA fragments migrate towards the positive electrode (the anode) within the gel. Due to the porous nature of the gel matrix, smaller DNA fragments move through more easily and therefore travel faster than larger fragments, effectively separating them based on size.