Final answer:
The ACTH-regulated enzymes for glucocorticoid production are located in the zona fasciculata of the adrenal cortex.
Step-by-step explanation:
The ACTH-regulated enzymes that produce glucocorticoids are located in the middle layer of the adrenal cortex, known as the zona fasciculata. This region is responsible for producing hormones like cortisol and is stimulated by adrenocorticotropic hormone (ACTH) secreted by the anterior pituitary gland. ACTH helps in the transport of cholesterol into the mitochondria where it is converted into pregnenolone and then into other adrenocortical hormones, including glucocorticoids.