Question

at about 47:45 min in the video, the remaining amounts of the sample to be separated was loaded onto the chromatography column using hexanes, instead of the eluting solvent of 9:1 hexanes : ethyl acetate, why?

Answer 1

The remaining amounts of the sample were loaded onto the chromatography column using hexanes instead of the eluting solvent of 9:1 hexanes : ethyl acetate, possibly due to reasons such as the eluting solvent not providing sufficient separation or the compound having better solubility in hexanes.

Step-by-step explanation:

At about 47:45 min in the video, the remaining amounts of the sample to be separated were loaded onto the chromatography column using hexanes instead of the eluting solvent of 9:1 hexanes : ethyl acetate. This could be due to several reasons:

• The eluting solvent might not have provided sufficient separation for the compound of interest.
• The compound might have better solubility in hexanes compared to the eluting solvent.
• The chromatographer might have wanted to explore different solvents to optimize the separation.