Final answer:
During initiation of transcription, the complex moves from a closed pre initiation complex to an open pre initiation complex upon the binding of transcription factors and RNA polymerase to the promoter region.
Step-by-step explanation:
The complex moves from a closed pre initiation complex to an open pre-initiation complex upon the binding of transcription factors and RNA polymerase to the promoter region. This complex, known as the initiation complex, helps to unwind the DNA double helix and create a transcription bubble, enabling RNA synthesis to occur.