Question

A Chimpanzee-derived RNA sequence forms a double loop between the bases CGU. Draw the structure, define structural differences between RNA and DNA, and explain the melting temperature (TM) of a DNA molecule and its influencing factors.

Answer 1

RNA forms a double loop between the bases of CGU, and there are structural differences between RNA and DNA. The melting temperature (TM) of a DNA molecule is influenced by factors such as DNA length, salt concentration, and the presence of other molecules.

Step-by-step explanation:

In RNA, the nucleotide sequence forms a double loop between the bases CGU. This means that the C base pairs with the G base, creating a stable structure. The structure of RNA is different from DNA in a few ways:

• Thymine (T) in DNA is replaced by uracil (U) in RNA.
• RNA is usually single-stranded, while DNA has a double-stranded helix structure.
• The sugar component in RNA is ribose, while DNA has deoxyribose sugar.

The melting temperature (TM) of a DNA molecule refers to the temperature at which the double-stranded DNA separates into individual single strands. The influencing factors of TM include:

• The length and sequence of the DNA.
• The concentration of salt ions in the solution.
• The presence of other molecules, such as proteins or small molecules, that can interact with the DNA.