Final answer:
In DNA sequencing, a run refers to the process of sequencing a DNA fragment using the chain termination method. The DNA fragment is cut into smaller pieces, labeled with a fluorescent dye, separated based on size, and the sequence of nucleotides is determined.
Step-by-step explanation:
In DNA sequencing, a run refers to the process of sequencing a DNA fragment using the chain termination method. The DNA fragment is cut into smaller pieces, and each piece is labeled with a fluorescent dye. Then, the fragments are separated based on their sizes using gel electrophoresis, and the sequence of nucleotides in each fragment is determined. This process is repeated multiple times, resulting in a series of sequences, or runs, that can be analyzed to obtain the complete DNA sequence.