Question

Describe one cycle of PCR including the 3 steps involved. be sure to include and underline the 4 main ingredients of PRC reaction.

Answer 1

The PCR cycle consists of denaturation, annealing, and DNA synthesis steps. The main ingredients in a PCR reaction are template DNA, primers, nucleotides, and polymerase.

Step-by-step explanation:

The polymerase chain reaction (PCR) consists of three steps: denaturation, annealing, and DNA synthesis. In the denaturation step, the DNA template is heated to break the hydrogen bonds and separate the double-stranded DNA into single strands. In the annealing step, the temperature is lowered and specific primers bind to the complementary regions of the DNA template. Finally, in the DNA synthesis step, the temperature is raised again and the enzyme Taq polymerase extends the primers, synthesizing new DNA strands.

The four main ingredients in a PCR reaction are:

• Template DNA: The DNA segment being amplified.
• Primers: Short DNA sequences that flank the target region and are complementary to it.
• Nucleotides: The building blocks used to synthesize new DNA strands.
• Polymerase: Enzyme, such as Taq polymerase, that catalyzes the DNA synthesis reaction.