Final answer:
The annealing step of PCR typically requires temperatures between 40-60°C for the primers to bind to the single-stranded DNA template, which is essential for the specificity and success of the amplification.
Step-by-step explanation:
The temperature range required for the annealing step of the Polymerase Chain Reaction (PCR) is typically between 40-60°C. Annealing is a critical step within the PCR process wherein primers bind to the single-stranded DNA template.
These primers need to attach precisely to the target sequences to ensure the specificity of the amplification. If the annealing temperature is too high, as in the case where it was mistakenly set to 65°C instead of around 50°C, the primers may not bind effectively to the target DNA, leading to reduced yield or specificity of the PCR product. Therefore, maintaining the correct annealing temperature is crucial for the success of a PCR experiment.