Final answer:
The support desk technician should instruct users to execute 'ipconfig /release' and then 'ipconfig /renew' on their Windows PCs to refresh their IP configuration and re-establish connection to the file server.
Step-by-step explanation:
When a router is replaced in a network, existing connections may still retain old routing information that needs to be refreshed. The support desk technician should instruct users to run the ipconfig /release followed by the ipconfig /renew command on their Windows PCs. These commands will release the current IP configuration and request a new lease from the DHCP server, which should include the updated route to access the file server. If the computer has a static IP, however, the commands will not work, and the network settings will need to be manually updated to ensure that the new route is included. It may also be beneficial to run the ipconfig /flushdns command to clear the DNS resolver cache in case the file server's address had been resolved previously and was cached.