Final answer:
Since the actual sequence reads are not provided, there is not enough information provided to determine if the short sequence reads from three different bacteria species form a single contig.
Step-by-step explanation:
To determine whether short sequence reads form a single contig, one must examine the overlaps between the sequences. A contig is a set of overlapping DNA segments that together represent a consensus region of DNA. In this question, we are provided with short sequence reads from three different bacteria species upstream of a conserved gene. A contig would be formed if there are sufficient overlaps that provide a continuous sequence across all reads. Unfortunately, the sequences themselves are not provided in the question, which means we lack the necessary information to answer whether the reads form a contig.
Therefore, the correct answer is D. Not enough information is given to determine whether all three short sequence reads generate a single contig or not.