Question

Nick translation by intact DNA pol I

1. add ____ __to nick DNA
2. Add one p-dNTP, three cold __ and ___
*nick moves _____ to ___

Answer 1

1. Add enzyme DNA Polymerase I to nick DNA.

2. Add one phosphorylated deoxynucleotide triphosphate (p-dNTP), three cold dNTPs, and enzyme DNA ligase; the nick moves 5' to 3'.

Step-by-step explanation:

Nick translation is a DNA labeling technique performed by DNA Polymerase I, which involves the removal of nucleotides and the simultaneous addition of labeled nucleotides to the 5' to 3' end of DNA, starting at a nick. To complete the student's sentence, the blanks should be filled as follows:

Nick translation is a process where DNA Polymerase I adds labeled nucleotides at a DNA nick. The process requires the enzyme to bind to the 5' end and add nucleotides to the 3' end, while enzyme DNA ligase joins the nicks.

DNA Polymerase I binds to the 5' end of the nick and adds nucleotides to the 3' end of the new strand as it hydrolyzes the 5' to 3' from the existing strand. DNA ligase then stitches these nicks, or Okazaki fragments, together.