Question

14. Where does RAG-1/2 cut the dsDNA depicted in the figure?

A. Between VL and the 7 bp regions
B. Between the 7 bp and 12 bp regions
C. Between the 12 bp and 9 bp regions
D. Within the 12 bp region
E. RAG-1/2 does not cut dsDNA

Answer 1

RAG-1/2 cuts the dsDNA is A. Between VL and the 7 bp regions.

Step-by-step explanation:

RAG-1/2 is an enzyme responsible for the rearrangement of immunoglobulin genes during the maturation of B cells. It cuts DNA at specific locations to facilitate gene recombination.

The region between VL (variable light chain gene segment) and the 7 bp regions is the appropriate location where RAG-1/2 cuts the dsDNA. This cleavage allows for the joining of the variable and joining segments, resulting in the formation of a unique variable chain.

RAG-1/2 does not cut within the 12 bp region or between the 12 bp and 9 bp regions, as these regions are not involved in the recombination process. Therefore the correct answer is A. Between VL and the 7 bp regions.