Final answer:
In eukaryotic transcription initiation, following the binding of TFIID to the TATA box, TFIIA and TFIIB are recruited. Subsequently, TFIIE, TFIIF, and TFIIH are added to the complex, which enables the binding of RNA polymerase II, leading to the phosphorylation and activation of the complex for mRNA synthesis.
Step-by-step explanation:
The process of eukaryotic transcription initiation begins with the assembly of a complex at the gene promoter. TFIID, which contains the TATA-binding protein (TBP), is one of the first factors to bind to the promoter's TATA box. Following TFIID, TFIIA and TFIIB are recruited to the promoter.
Subsequently, additional transcription factors, including TFIIE, TFIIF, and TFIIH, join the complex. These factors, along with several other initiation factors, support the binding of RNA polymerase II and contribute to the formation of the transcription initiation complex. Phosphorylation of RNA polymerase II occurs at this stage, which triggers the release of some transcription factors from the complex, positioning RNA polymerase II to begin the synthesis of mRNA.