Question

For each pair of amino acids, identify which will be eluted first?

Answer 1

The order of elution from the column depends on the polarity of the amino acids, with more polar amino acids eluting later and less polar amino acids eluting earlier.

Step-by-step explanation:

The order of elution from the column depends on the polarity of the amino acids. Amino acids with higher polarity will be more attracted to the stationary phase and will elute later, while amino acids with lower polarity will be less attracted and will elute earlier.

For example, amino acids like serine, threonine, and tyrosine have hydroxyl (-OH) groups which increase their polarity and make them more attracted to the stationary phase. These amino acids will elute later.

In contrast, amino acids like alanine, valine, and leucine have hydrophobic side chains which decrease their polarity and make them less attracted to the stationary phase. These amino acids will elute earlier.