Final answer:
Substrate phosphorylation in glycolysis results in the phosphorylation of ADP to form ATP, which occurs during the energy payoff phase and is catalyzed by enzymes such as phosphoglycerate kinase. The correct option is 1.
Step-by-step explanation:
The result of substrate phosphorylation in glycolysis is that ADP is phosphorylated to form ATP. This is a direct method of phosphorylation where a phosphate group is transferred from an intermediate reactant in the pathway to ADP, thereby generating ATP.
This process occurs during the energy payoff phase of glycolysis, where enzymes like phosphoglycerate kinase catalyze the transfer of high-energy phosphate from 1,3-bisphosphoglycerate to ADP, forming ATP.