Question

RNA interference is a mechanism of gene silencing that is mediated by the presence of double-stranded RNA. Arrange the steps involved in gene silencing by RNAi.

1) Activation of RNA-induced silencing complex (RISC)
2) Cleavage of target mRNA by RISC
3) Binding of small interfering RNA (siRNA) to RISC
4) Degradation of target mRNA by RISC

Answer 1

The steps involved in gene silencing by RNAi are binding siRNA to RISC, activation of RISC, cleavage of target mRNA by RISC, and degradation of target mRNA by RISC.

Step-by-step explanation:

The steps involved in gene silencing by RNA interference (RNAi) are:

1. Binding of small interfering RNA (siRNA) to RNA-induced silencing complex (RISC)
2. Activation of RNA-induced silencing complex (RISC)
3. Cleavage of target mRNA by RNA-induced silencing complex (RISC)
4. Degradation of target mRNA by RNA-induced silencing complex (RISC)

The process starts with the binding of siRNA to RISC, which activates RISC. Then, RISC cleaves the target mRNA, leading to its degradation.