Final answer:
The nurse should inject 0.1mL of purified protein derivative as part of the tuberculin skin test. This test assesses exposure to tuberculosis through a delayed hypersensitivity reaction, with a positive result indicating an immune response to the bacterium. BCG vaccination could cause false-positive results.
Step-by-step explanation:
A nurse preparing to administer a tuberculin skin test should take the action of injecting 0.1mL of purified protein derivative into the skin of the clients. The injection should produce a raised wheal initially, and the test must be read within 48-72 hours. A positive result is indicated by redness, swelling, or hardness; the size of the responding region is measured to determine the final result.
The tuberculin skin test uses bacterial proteins from Mycobacterium tuberculosis to test for delayed hypersensitivity. A positive result indicates that the patient has been exposed to the bacteria and has a cellular immune response against it. However, in areas where the BCG vaccine is used, a positive tuberculin skin test might reflect prior vaccination rather than an active infection.