Question

What is the classical NF-kappa-B target sequence?

a. 5'-GGGRNNYYCC-3'
b. 5'-GGGAYRTCCC-3'
c. 5'-AGGGRNNYYC-3'
d. 5'-GGGARNNYYC-3'

Answer 1

The correct classical NF-kappa-B target sequence is 5'-GGGACTTTCC-3'. NF-kappa-B binds to this sequence to induce gene expression involved in various cellular processes.

Step-by-step explanation:

The classical NF-kappa-B target sequence is a specific DNA motif recognized by the NF-kappa-B transcription factor complex. Among the options presented, the correct sequence is option b. 5'-GGGACTTTCC-3', also represented as 5'-GGGAYRTCCC-3', in which 'Y' represents pyrimidines (C or T) and 'R' represents purines (A or G). This sequence is crucial for the binding of NF-kappa-B to DNA, which in turn is essential for the activation of various genes involved in immune response, cell proliferation, and survival.

NF-kappa-B induces gene expression by being activated in response to various stimuli, such as cytokines, free radicals, ultraviolet irradiation, oxidized LDL, and bacterial or viral antigens. Once activated, NF-kappa-B translocates into the nucleus where it binds to its target sequence in the promoters or enhancers of responsive genes, promoting the transcription of these genes and ultimately resulting in protein production.