Final answer:
Spo11 actually creates double-strand breaks in DNA during meiosis and is then removed, allowing the 5' ends to be resected to produce 3'-protruding ends; thus, the correct answer is False.
Step-by-step explanation:
The enzyme Spo11 plays a crucial role in the process of meiosis. Contrary to the statement in the question, Spo11 does not digest the 5' end to generate 3'-protruding ends. In fact, Spo11 functions by creating double-strand breaks (DSBs) in the DNA during meiotic recombination. After the DSBs are introduced, the enzyme is removed from the DNA, and the 5' ends are resected to generate 3'-protruding ends. These 3' ends invade the homologous chromosome to begin the process of genetic recombination. Therefore, the correct answer to the question is False.