Question

This technique allows the amplification of DNA sequences:

A Polymerase chain reaction (PCR)
B Cloning into bacteria
C Incorporation of dideoxynucleotides
D A & B
E A & C

Answer 1

Polymerase Chain Reaction (PCR) is the technique that allows DNA sequences to be amplified, producing millions of copies of a target DNA sequence from a small amount of starting material.

Step-by-step explanation:

The technique that allows the amplification of DNA sequences is known as the Polymerase Chain Reaction (PCR). PCR enables scientists to generate millions of copies of a specific DNA region in just a few hours. This process uses a thermal cycler to repeatedly cycle through series of temperature changes which are: denaturing the DNA, annealing primers to the sequences to be amplified, and extending the primers to form a new DNA strand with the help of Taq polymerase. Taq polymerase is a special enzyme that is both heat-stable and capable of assembling new DNA strands as it withstands the high temperatures necessary for PCR. The entire PCR process can rapidly produce large quantities of a target DNA segment from a very small amount of starting material, and is fundamental in fields such as medical diagnostics, forensic science, and biological research.