Lack of oxygen, presence of alternative electron acceptors, or production of fermentation products indicate anaerobic respiration.
Here are some indications that the respiration reactions occurring in a bacterium are anaerobic:
- Absence of oxygen: This is the most obvious indication. If the bacterium is not exposed to oxygen, it will not be able to carry out aerobic respiration.
- Presence of alternative electron acceptors: In the absence of oxygen, some bacteria can use other molecules as electron acceptors in their respiration reactions. These alternative electron acceptors can include nitrate, sulfate, or fumarate.
- Production of fermentation products: Fermentation is a type of anaerobic respiration that produces organic waste products, such as lactate, ethanol, or carbon dioxide. If the bacterium is producing these products, it is a strong indication that it is carrying out anaerobic respiration.
- Lack of a cytochrome oxidase enzyme: Cytochrome oxidase is an enzyme that is essential for aerobic respiration. If the bacterium does not have this enzyme, it will not be able to carry out aerobic respiration.
- Growth in an oxygen-free environment: Some bacteria are obligate anaerobes, which means that they can only grow in the absence of oxygen. If the bacterium can grow in an oxygen-free environment, it is a strong indication that it is an anaerobe.
Here are some specific examples of bacteria that carry out anaerobic respiration:
- Lactic acid bacteria: These bacteria are responsible for the fermentation of milk into yogurt and cheese. They produce lactate as a fermentation product.
- Clostridium botulinum: This bacterium is responsible for botulism, a serious foodborne illness. It produces botulinum toxin, which is one of the deadliest toxins known.
- Desulfovibrio desulfuricans: This bacterium is responsible for the reduction of sulfate to sulfide in the environment. It produces hydrogen sulfide as a waste product.
By looking for these clues, scientists can determine whether or not a bacterium is carrying out anaerobic respiration.