Final answer:
To prepare a 50 mL, 8% cell suspension from packed cells, carefully decant the supernatant and add sterile saline solution to the packed cells until the volume reaches 50 mL.
Step-by-step explanation:
To prepare a 50 mL, 8% cell suspension from packed cells, you will need to follow the steps below: Obtain the packed cells and allow them to settle down to the bottom of the container. Decant or remove the supernatant (the liquid on top) carefully, without disrupting the packed cells.
Add sterile saline solution slowly and gently to the packed cells. Continuously mix the suspension to ensure an even distribution of cells. Continue adding sterile saline until the volume reaches 50 mL.
By following these steps, you will obtain a 50 mL cell suspension with approximately 8% concentration. Remember to use sterile techniques to maintain the purity of the suspension.